Protocol library
SinglePreclinicalPreclinicalVery Low confidenceSourced

TB-500

A synthetic fragment marketed by association with thymosin beta-4. Full-length thymosin beta-4 findings do not establish an injectable TB-500 protocol.

Evidence context, not a personal protocol

Quantities below reproduce their stated source context—label, human study, laboratory work or educational guide. They do not assess suitability, product equivalence or individual risk.

Community-reported schedule

Community-reported · not a validated schedule

Community dose schedule

Two community-provenance subcutaneous lines - a loading-then-maintenance pattern from a protocol author and an educational fragment titration. Community reports only, not a validated or trial-verified schedule.

Evidence source
Reported maximum quantityChoose the maximum quantity reported by this community line. Any earlier phase can be selected below for its arithmetic.

Reported quantities by phase

Select a point for its calculation examples
  1. Acute (3x/day)
    650 mcg
    Per-injection acute anecdotes span roughly 350-650 mcg up to three times daily, dose-matched to BPC-157.
  2. Maintenance (2x/day)
    350 mcg
    Step down for maintenance

Vial-aware arithmetic

Reconstitution examples, up to 3 mL

Choose the vial quantity and BAC water first. The diagram and results below update for the selected 650 mcg reported quantity. Higher-strength vials may naturally land outside the 20-30 unit band.

Vial quantity

Choose the quantity printed on the vial.

BAC water added

Use 1, 2 or 3 mL, or enter a custom volume up to 3 mL.

Open the full reverse calculator
Three millilitre research vial, diluent vial and capped U-100 syringe on an ink-blue surface
One small vial, one measured diluent volume and one U-100 scale. The arithmetic connects all three.
BAC water selected
2 mL

Basic example limit: 3 mL maximum. Check the actual vial and product-specific limits.

1 mL BAC
13 units

resulting U-100 draw

Concentration
5 mg/mL
BAC added
1 mL
3 mL BAC
39 units

resulting U-100 draw

Concentration
1.667 mg/mL
BAC added
3 mL

Formula: draw units = selected mg × BAC mL ÷ vial mg × 100. The 20-30 unit band is a measurement reference, not a recommendation. Results are mathematical examples, not preparation instructions.

Dosage and protocol evidence

Each card states what the quantities are based on. A third-party or animal schedule is not a validated human dose.

Community GuideSubcutaneous in guideDaily
Third-party educational TB-500 schedule

500 mcg daily in weeks 1-2, 600 mcg in weeks 3-4, 750 mcg in weeks 5-8 and 1 mg in weeks 9-12

No controlled human TB-500 fragment study validates this schedule. Full-length thymosin beta-4 evidence must not be used as confirmation.

View source
Community GuideSubcutaneous in reportsDaily for the fragment schedules
online research community fragment 17-23 discussion

Two supplier-derived fragment schedules are 750 mcg daily for 20 days or 450 mcg daily for 30 days

The same channel also repeats a 5 mg twice-weekly schedule likely inherited from full-length thymosin beta-4. The archive repeatedly warns that TB-500, full TB4 and fragments are conflated; raw/peptide-education/pe-tb-500-frag.md:18-32 and 378.

View source
Community GuideSubcutaneous in reportsUp to 3x daily acute, reduced for maintenance
authenticated research-forum (Peppys) reports and a protocol author

Subcutaneous acute-injury anecdotes cluster around 350-650 mcg three times daily, stepping down to about 350 mcg twice daily for maintenance; reference charts list 450-750 mcg subcutaneously daily for 20-30 days, and an old long-acting form is dosed 1-2 mg two to three times weekly

Forum provenance from an authenticated archive with usernames omitted. The archive claims that what is sold today as TB-500 is actually short-acting TB4, which it uses to justify frequent daily dosing; that identity claim is recorded as a community assertion, not a validated equivalence, and the TB-500 fragment is kept separate from full-length thymosin beta-4.

View source

Evidence summary

A synthetic fragment marketed by association with thymosin beta-4. Full-length thymosin beta-4 findings do not establish an injectable TB-500 protocol.

Protocol basis

The central evidence decision is molecule identity: a protocol cannot be imported from a different, full-length protein with different formulation and exposure.

Community provenance (Peppys)

An authenticated research-forum archive dose-matches TB-500 to BPC-157: subcutaneous acute-injury anecdotes cluster around 350-650 mcg up to three times daily, stepping down to about 350 mcg twice daily for maintenance, usually stacked with BPC-157. The archive asserts that material sold today as TB-500 is actually short-acting TB4 and uses that claim to justify frequent daily dosing; that equivalence is recorded as a community claim rather than validated fact, and the TB-500 fragment is kept separate from full-length thymosin beta-4. Usernames and post numbers are omitted. The planner above reproduces the arithmetic of these reported quantities; it does not validate them.

What remains uncertain

Human pharmacokinetics, dose-response, manufacturing standards and controlled outcomes are not established.

Warnings and contraindications

TB-500 fragment identity must not be conflated with full-length thymosin beta-4.
No adequate controlled human injection regimen for TB-500 was identified.
Animal wound-healing experiments do not establish human safety or effectiveness.
Organised research bench with labelled storage tray, vial rack, notebook and thermometer
A clean workspace supports traceability. It does not establish sterility.

Shared across protocols

Supplies and general handling

Keep the universal checklist short. Product-specific labelling and source documentation always take priority over a general guide.

Research vial

Select the labelled vial quantity used in the calculation.

View Peppys vial

Bacteriostatic water

Use only a diluent appropriate to the research method and formulation.

View Peppys BAC water

U-100 measuring syringe

Use a new sterile device for every entry. Source locally.

Source locally

Wipes and sharps container

Plan clean handling and immediate sharps disposal. Source locally.

Source locally

Storage follows the label

Do not apply a universal refrigerator rule. Follow the product, diluent and study documentation for temperature, light and expiry.

Use sterile equipment once

CDC injection-safety guidance calls for a new sterile needle and syringe for each injection and entry into a medication container.

Stability is formulation-specific

Do not infer a post-mixing lifetime from BAC water alone. Discard material when identity, integrity or sterility is in doubt.