Protocol library
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Semax

A synthetic ACTH fragment analogue studied mainly in Russian-language and intranasal contexts. Evidence does not map cleanly to a reconstituted injectable vial.

Evidence context, not a personal protocol

Quantities below reproduce their stated source context—label, human study, laboratory work or educational guide. They do not assess suitability, product equivalence or individual risk.

Community-reported schedule

Community-reported · not a validated schedule

Community dose schedule

A community-provenance subcutaneous line from an authenticated research forum - roughly 100 to 250 mcg daily. The archive's common route is intranasal, shown separately. Community reports only, not a validated schedule.

Evidence source
Reported maximum quantity
100 to 250 mcg daily SubQ (forum)
Choose the maximum quantity reported by this community line. Any earlier phase can be selected below for its arithmetic.

Reported quantities by phase

Select a point for its calculation examples
  1. Lower reports (SubQ)
    100 mcg
    Nasal is the common route; a vendor chart lists 100 mcg (0.1 mL) subcutaneously daily. Dose early in the day to limit insomnia.
  2. Higher reports
    250 mcg
    +150 mcg
    Upper cluster near the ~250 mcg-per-spray figure; one report ran 500 mcg/day intranasally on 20-on/10-off. Intranasal dominates the archive.

Vial-aware arithmetic

Reconstitution examples, up to 3 mL

Choose the vial quantity and BAC water first. The diagram and results below update for the selected 100 mcg reported quantity. Higher-strength vials may naturally land outside the 20-30 unit band.

Vial quantity

Choose the quantity printed on the vial.

BAC water added

Use 1, 2 or 3 mL, or enter a custom volume up to 3 mL.

Open the full reverse calculator
Three millilitre research vial, diluent vial and capped U-100 syringe on an ink-blue surface
One small vial, one measured diluent volume and one U-100 scale. The arithmetic connects all three.
BAC water selected
3 mL

Basic example limit: 3 mL maximum. Check the actual vial and product-specific limits.

1 mL BAC
2 units

resulting U-100 draw

Concentration
5 mg/mL
BAC added
1 mL
2 mL BAC
4 units

resulting U-100 draw

Concentration
2.5 mg/mL
BAC added
2 mL

Formula: draw units = selected mg × BAC mL ÷ vial mg × 100. The 20-30 unit band is a measurement reference, not a recommendation. Results are mathematical examples, not preparation instructions.

Dosage and protocol evidence

Each card states what the quantities are based on. A third-party or animal schedule is not a validated human dose.

Formulation MathIntranasalPer verified spray
Worked intranasal formulation using a verified 0.10 mL actuator

A 5 mg vial at a measured 5 mL final volume yields 1 mg/mL and 100 mcg per spray; 10 mL yields 0.5 mg/mL and 50 mcg per spray

This is concentration and actuator arithmetic, not a universal dose. Pump output, vehicle, priming, stability and the exact formulation must be independently established.

View source
Human StudyIntranasalStudy-specific, from single exposure to daily for 10 days
Published human intranasal experiments summarised by the source

Reported protocols ranged from single 0.25-1.2 mg exposures to a condition-specific 6 mg daily, 10-day post-stroke course

These distinct studies used different populations and formulations. Their quantities should be read individually rather than combined into one schedule.

View source
Community GuideIntranasal or subcutaneous in reportsUsually divided daily; cycle varies
online research community summaries and reports

The spotlight states 300-600 mcg daily divided intranasally or subcutaneously; other channel summaries span 750-1,000 mcg intranasally daily or 100-300 mcg subcutaneously daily

Condition-specific intranasal schedules in the same channel rise to 6-12 mg daily for 10 days. Route equivalence and cycling are not established, so these ranges must not be merged into one protocol; raw/peptide-education/pe-semax.md:18-29 and 53-78.

View source
Community GuideIntranasal or subcutaneous in reportsUsually early-day; some 20-on/10-off cycling
authenticated research-forum (Peppys) reports and vendor dosing charts

Semax is used mostly intranasally at roughly 100 mcg per nostril up to about 250 mcg per spray, dosed early in the day; a vendor chart also lists 100 mcg (0.1 mL) subcutaneously daily, and one report ran 500 mcg per day intranasally on a 20-days-on, 10-days-off cycle. N-Acetyl Semax Amidate is run at a lower dose as a more potent variant

Forum provenance with usernames omitted. Intranasal is the dominant route and experts insist it be reconstituted with nasal saline rather than bacteriostatic water; intranasal and subcutaneous quantities are kept separate rather than interconverted.

View source

Evidence summary

A synthetic ACTH fragment analogue studied mainly in Russian-language and intranasal contexts. Evidence does not map cleanly to a reconstituted injectable vial.

Protocol basis

This page reports the formulation mismatch as part of the protocol: published intranasal work is not converted into injectable quantities.

Community provenance (Peppys)

An authenticated research-forum archive uses Semax mainly as an intranasal nootropic, clustering around 100 mcg per nostril up to about 250 mcg per spray and dosed early in the day to limit insomnia. A vendor chart in the same archive lists a 100 mcg subcutaneous daily figure, and one report ran 500 mcg per day intranasally on a 20-days-on, 10-days-off cycle; the more potent N-Acetyl Semax Amidate is run at a lower dose. Experts insist intranasal Semax be reconstituted with nasal saline rather than bacteriostatic water, and the intranasal and subcutaneous quantities are kept separate rather than interconverted. Usernames are omitted; the planner above reflects only the subcutaneous line while the archive's common route is intranasal, and it reproduces the arithmetic without validating it.

What remains uncertain

Independent replication, route-specific bioavailability, long-term safety and equivalence to research products remain uncertain.

Warnings and contraindications

Most clinical use and research concerns intranasal formulations in limited jurisdictions.
Intranasal findings do not establish an injectable protocol.
Internationally accessible controlled evidence and product equivalence are limited.
Organised research bench with labelled storage tray, vial rack, notebook and thermometer
A clean workspace supports traceability. It does not establish sterility.

Shared across protocols

Supplies and general handling

Keep the universal checklist short. Product-specific labelling and source documentation always take priority over a general guide.

Research vial

Select the labelled vial quantity used in the calculation.

View Peppys vial

Bacteriostatic water

Use only a diluent appropriate to the research method and formulation.

View Peppys BAC water

U-100 measuring syringe

Use a new sterile device for every entry. Source locally.

Source locally

Wipes and sharps container

Plan clean handling and immediate sharps disposal. Source locally.

Source locally

Storage follows the label

Do not apply a universal refrigerator rule. Follow the product, diluent and study documentation for temperature, light and expiry.

Use sterile equipment once

CDC injection-safety guidance calls for a new sterile needle and syringe for each injection and entry into a medication container.

Stability is formulation-specific

Do not infer a post-mixing lifetime from BAC water alone. Discard material when identity, integrity or sterility is in doubt.