1 mL BAC
4 units
resulting U-100 draw
- Concentration
- 5 mg/mL
- BAC added
- 1 mL
SingleInvestigationalPhase 2Low confidenceSourced
LL-37
A human antimicrobial peptide studied clinically as a topical wound product. Controlled evidence does not validate systemic or subcutaneous injection.
Evidence context, not a personal protocol
Quantities below reproduce their stated source context—label, human study, laboratory work or educational guide. They do not assess suitability, product equivalence or individual risk.
Community-reported schedule
Community-reported · not a validated scheduleCommunity dose schedule
A single community-provenance subcutaneous line from an authenticated forum - a curated 100-300 mcg per-application range, with no member self-dose captured. Community reference only, not a validated or trial-verified schedule.
Reported maximum quantity
100-300 mcg per application (curated)
Choose the maximum quantity reported by this community line. Any earlier phase can be selected below for its arithmetic.Reported quantities by phase
Select a point for its calculation examples- Per application (SubQ)100-300 mcgA curated admin range gives 100-300 mcg per application near infection; no member self-dose was captured and topical use is kept separate.
Vial-aware arithmetic
Reconstitution examples, up to 3 mL
Choose the vial quantity and BAC water first. The diagram and results below update for the selected 100-300 mcg reported quantity. Higher-strength vials may naturally land outside the 20-30 unit band.
BAC water selected
3 mL
Basic example limit: 3 mL maximum. Check the actual vial and product-specific limits.
U-100 draw12 U
20-30 U reference band
2 mL BAC
8 units
resulting U-100 draw
- Concentration
- 2.5 mg/mL
- BAC added
- 2 mL
3 mL BAC
12 units
resulting U-100 draw
- Concentration
- 1.667 mg/mL
- BAC added
- 3 mL
Shown in the diagram. Closest of these examples to 25 units.
Formula: draw units = selected mg × BAC mL ÷ vial mg × 100. The 20-30 unit band is a measurement reference, not a recommendation. Results are mathematical examples, not preparation instructions.
Dosage and protocol evidence
Each card states what the quantities are based on. A third-party or animal schedule is not a validated human dose.
Human StudyTopicalTwice weekly
Adults with hard-to-heal venous leg ulcers
Topical 0.5 mg/mL or 1.6 mg/mL LL-37 applied twice weekly for 13 weeks
The phase 2b study combined topical study product with compression therapy; this is not an injectable protocol.
View sourceCommunity GuideSubcutaneous in spotlightDaily for four weeks in spotlight
online research community summary
The channel states 100-300 mcg per dose, usually subcutaneously near infection or injury, daily for four weeks followed by a washout
The only human trial discussed in the same channel is topical. The systemic schedule is a community spotlight claim and is not validated by the cited wound studies; raw/peptide-education/pe-ll-37.md:18-21 and 67-68.
View sourceCommunity GuideSubcutaneous or topical in referencesPer application; shorter acute courses
authenticated research-forum (Peppys) LL-37 curated references
A curated administrator range describes 100-300 mcg per application subcutaneously near the site of infection, with a separate topical concentration of 0.5-1.6 mg/mL; a clinician chart lists a 2000 mcg/mL injectable whose dose varies by indication
Forum provenance from an authenticated archive with usernames omitted. No individual member logged a self-administered quantity, so these are curated reference figures rather than personal reports; the injectable and topical routes are kept separate and the archive cautions against large or prolonged dosing.
View sourceEvidence summary
A human antimicrobial peptide studied clinically as a topical wound product. Controlled evidence does not validate systemic or subcutaneous injection.
Protocol basis
The strongest protocol is topical and wound-specific. The page preserves concentration, frequency and compression context but does not translate them to injection.
Community provenance (Peppys)
An authenticated research-forum archive carries no member-authored LL-37 dosing log; the only figures are curated administrator and chart references. Those describe roughly 100-300 mcg per subcutaneous application near a site of infection, alongside a separate topical concentration of 0.5-1.6 mg/mL that is not interconverted with the injectable amount. The archive is explicit that these are reference values rather than personal reports, and it cautions against large or prolonged dosing. Usernames and post numbers are omitted and the figures are logged as community provenance without endorsement. The planner above reproduces the arithmetic of these reported quantities; it does not validate them.
What remains uncertain
Systemic safety, subcutaneous pharmacokinetics and benefit outside topical wound care are not established.
Warnings and contraindications
The human trials applied LL-37 topically to venous leg ulcers.
Topical concentrations cannot be converted into systemic injection quantities.
The larger phase 2b trial did not improve healing in the full study population.
Shared across protocols
Supplies and general handling
Keep the universal checklist short. Product-specific labelling and source documentation always take priority over a general guide.
Research vial
Select the labelled vial quantity used in the calculation.
View Peppys vialBacteriostatic water
Use only a diluent appropriate to the research method and formulation.
View Peppys BAC waterU-100 measuring syringe
Use a new sterile device for every entry. Source locally.
Source locallyWipes and sharps container
Plan clean handling and immediate sharps disposal. Source locally.
Source locallyStorage follows the label
Do not apply a universal refrigerator rule. Follow the product, diluent and study documentation for temperature, light and expiry.
Use sterile equipment once
CDC injection-safety guidance calls for a new sterile needle and syringe for each injection and entry into a medication container.
Stability is formulation-specific
Do not infer a post-mixing lifetime from BAC water alone. Discard material when identity, integrity or sterility is in doubt.