1 mL BAC
2 units
resulting U-100 draw
- Concentration
- 5 mg/mL
- BAC added
- 1 mL
SingleInvestigationalEarly HumanLow confidenceSourced
DSIP
A small neuropeptide investigated in older and heterogeneous sleep experiments. The literature does not support a current standard administration protocol.
Evidence context, not a personal protocol
Quantities below reproduce their stated source context—label, human study, laboratory work or educational guide. They do not assess suitability, product equivalence or individual risk.
Community-reported schedule
Community-reported · not a validated scheduleCommunity dose schedule
A community-provenance subcutaneous line from forum reports - starting near 100 mcg in the evening and titrated toward 250-500 mcg. Community reports only; the cited human studies used far larger IV morning doses, not reproduced here.
Reported maximum quantity
Evening titration (forum)
Choose the maximum quantity reported by this community line. Any earlier phase can be selected below for its arithmetic.Reported quantities by phase
Select a point for its calculation examples- Starting (evening)100 mcgReports begin near 100 mcg (some 50 mcg), subcutaneously or intranasally, one to two hours before bed or earlier because it can feel stimulating.
- Titrated (evening)250 mcg+150 mcgTitrated quantities cluster around 250-500 mcg; response is highly variable, with many reporting no effect even above 450 mcg.
Vial-aware arithmetic
Reconstitution examples, up to 3 mL
Choose the vial quantity and BAC water first. The diagram and results below update for the selected 100 mcg reported quantity. Higher-strength vials may naturally land outside the 20-30 unit band.
BAC water selected
3 mL
Basic example limit: 3 mL maximum. Check the actual vial and product-specific limits.
U-100 draw6 U
20-30 U reference band
2 mL BAC
4 units
resulting U-100 draw
- Concentration
- 2.5 mg/mL
- BAC added
- 2 mL
3 mL BAC
6 units
resulting U-100 draw
- Concentration
- 1.667 mg/mL
- BAC added
- 3 mL
Shown in the diagram. Closest of these examples to 25 units.
Formula: draw units = selected mg × BAC mL ÷ vial mg × 100. The 20-30 unit band is a measurement reference, not a recommendation. Results are mathematical examples, not preparation instructions.
Dosage and protocol evidence
Each card states what the quantities are based on. A third-party or animal schedule is not a validated human dose.
Community GuideSubcutaneous in guideDaily
Third-party educational schedule for a 5 mg research vial
100 mcg daily in week 1, 150 mcg in week 2, 200 mcg in week 3 and 250-300 mcg daily in weeks 4-8
This schedule is not reproduced by a modern controlled human trial. Historical DSIP studies were small and heterogeneous, so confidence remains very low.
View sourceCommunity GuideSubcutaneous or intranasal in reportsNear sleep in most reports; no consensus
online research community sleep reports
Reported quantities cluster around 100-400 mcg near sleep, including 250-500 mcg nightly and 300 mcg subcutaneously nightly
Cycling varies from three times weekly with month-long breaks to 30 days on and 30 days off. Several users report no measurable benefit, and one moved use before 6 PM because it felt stimulating; raw/peptide-education/pe-tesamorelin.md:2663-2728 and raw/bioregulators/bio-pinealon.md:289-293.
View sourceCommunity GuideSubcutaneous or intranasal in reportsEvening near sleep; nightly to a few times weekly
authenticated research-forum (Peppys) sleep reports
Subcutaneous or intranasal quantities cluster around 100 mcg in the evening titrated toward 250-500 mcg, dosed one to two hours before bed or as early as 5-6 PM because it can feel stimulating; a contested minority runs mg-range subcutaneous doses of roughly 1.5-2 mg (occasionally 5 mg) to cross a reported non-response threshold
Forum provenance from an authenticated archive with usernames omitted. The archive flags a direct conflict between practice and the cited human studies, which used about 25 nmol/kg intravenously (roughly 1.7 mg) in the morning, and records highly variable response with many reporting no effect even above 450 mcg. Community provenance, not controlled human dose finding; subcutaneous, intranasal and intravenous quantities are kept separate.
View sourceEvidence summary
A small neuropeptide investigated in older and heterogeneous sleep experiments. The literature does not support a current standard administration protocol.
Protocol basis
The evidence level reflects historical human exposure, but the absence of a coherent replicated schedule keeps confidence low.
Community provenance (Peppys)
An authenticated research-forum archive records subcutaneous or intranasal quantities clustering around 100 mcg in the evening, titrated toward roughly 250-500 mcg, dosed one to two hours before bed or earlier because some report a stimulating effect. The same archive flags a direct conflict with the cited human studies, which used about 25 nmol/kg intravenously in the morning (roughly 1.7 mg), and a minority who run mg-range subcutaneous doses to cross a reported non-response threshold. Response is described as highly variable, with many reporting no measurable benefit even above 450 mcg; usernames are omitted and figures are logged as community provenance without endorsement. The planner above reproduces the arithmetic of the low-dose subcutaneous reports only and does not validate them.
What remains uncertain
A validated indication, modern controlled efficacy, optimal route and product equivalence are not established.
Warnings and contraindications
Human studies are old, small and methodologically heterogeneous.
Sleep effects were inconsistent and do not establish a routine protocol.
Modern formulation, pharmacokinetic and long-term safety data are lacking.
Shared across protocols
Supplies and general handling
Keep the universal checklist short. Product-specific labelling and source documentation always take priority over a general guide.
Research vial
Select the labelled vial quantity used in the calculation.
View Peppys vialBacteriostatic water
Use only a diluent appropriate to the research method and formulation.
View Peppys BAC waterU-100 measuring syringe
Use a new sterile device for every entry. Source locally.
Source locallyWipes and sharps container
Plan clean handling and immediate sharps disposal. Source locally.
Source locallyStorage follows the label
Do not apply a universal refrigerator rule. Follow the product, diluent and study documentation for temperature, light and expiry.
Use sterile equipment once
CDC injection-safety guidance calls for a new sterile needle and syringe for each injection and entry into a medication container.
Stability is formulation-specific
Do not infer a post-mixing lifetime from BAC water alone. Discard material when identity, integrity or sterility is in doubt.